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Viral packaging was done in HEK-293 T cells just after cotransfection of your PSMD9 overexpression or shRNA plasmid Using the packaging plasmid psPAX2 along with the envelope plasmid pMD2.Just after incubating for 24 or forty eight h, the nonmigrated or noninvaded cells ended up Carefully removed from the upper chamber using a cotton swab. The ce